Voltage-sensitive dyes. Discerning contraction and electrical signals in myocardium
نویسندگان
چکیده
منابع مشابه
Optical signals from neurons with internally applied voltage-sensitive dyes.
We carried out experiments to monitor optically the generation and spread of action potentials and subthreshold potentials in the processes of individual neurons in ganglia of the snail, Helix aspersa. The neurons were selectively stained by intracellular pressure injection of voltage-sensitive dyes. Optical signals were detected by a system for fast, multiple-site optical monitoring, utilizing...
متن کاملUse of Voltage - Sensitive Dyes and Optical
-Understanding the spatio-temporal features of the information processing occurring in any complex neural structure requires the monitoring and analysis of the activity in populations of neurons. Electrophysiological and other mapping techniques have provided important insights into the function of neural circuits and neural populations in many systems. However, there remain limitations with th...
متن کاملPalette of fluorinated voltage-sensitive hemicyanine dyes.
Optical recording of membrane potential permits spatially resolved measurement of electrical activity in subcellular regions of single cells, which would be inaccessible to electrodes, and imaging of spatiotemporal patterns of action potential propagation in excitable tissues, such as the brain or heart. However, the available voltage-sensitive dyes (VSDs) are not always spectrally compatible w...
متن کاملImaging cortical electrical stimulation in vivo: fast intrinsic optical signal versus voltage-sensitive dyes.
We applied high-temporal-resolution optical imaging utilizing both the fast intrinsic optical signal (fIOS) and voltage-sensitive dyes (VSDs) to observe the spatiotemporal characteristics of rat somatosensory cortex during electrical stimulation. We find that changes in both the fIOS and VSD signals occur rapidly (<30 ms) after the stimulus is applied, suggesting that both membrane depolarizati...
متن کاملA computational model of the signals in optical imaging with voltage-sensitive dyes
Optical imaging with voltage-sensitive dyes (VSD imaging) can record neural activity over an area of several square centimeters with high spatiotemporal resolution. The relative contributions of subthreshold and suprathreshold activity to the VSD signal are, however, poorly understood, making it difficult to interpret the imaging result. This paper shows how the activity in a computational mode...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1982
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(82)84481-0